Effect of fish oil on intestinal Paneth cells in mouse with abdominal infection / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To investigate the effect of fish oil on intestinal Paneth cells in mouse with abdominal infection.</p><p><b>METHODS</b>Fifty C57BL/6J mouse were randomly divided into five groups (n=10 each): control group, sham group, infection group (cecal ligation and puncture, CLP), fish oil group (0.4 g/kg fish oil, intragastric administration every day, FO) and long chain triglyceride group (0.4 g/kg soybean oil, intragastric administration every day, LCT). The mouse were sacrificed and the terminal ileum was collected for lysozyme, cryptdin 4 and secreted phosphatidase A2 (sPLA2) analysis at the fourth day after operation. The changes of mouse body weight and intestinal mucosa pathology were observed.</p><p><b>RESULTS</b>The body weight, the mRNA levels of lysozyme, cryptdin 4 and sPLA2 and the protein level of lysozyme of Paneth cells in the infection group were reduced compared with the control group (0.78±0.34 vs. 1.83±0.11, 0.99±0.44 vs. 2.02±0.33, 0.92±0.25 vs. 1.50±0.27, 0.31±0.06 vs. 0.45±0.05, all P<0.05), meanwhile the intestinal villi collapse and breakage occurred obviously. Fish oil could up-regulate the mRNA and protein expression of lysozyme (1.23±0.27 vs. 0.78±0.34 and 0.62±0.23, 0.38±0.07 vs. 0.31±0.06 and 0.32±0.06, all P<0.05) and alleviate the mucosa injury compared with the infection group and LCT group.</p><p><b>CONCLUSIONS</b>The function of intestinal Paneth cells is damaged apparently after cecal ligation and puncture. Fish oil can relieve this injury.</p>

Analysis of Prescribing Patterns in Outpatients based on the Latest Chinese Guideline for the Management of Hypertension / 天津医药

Objective To investigate the pattern of antihypertensive medication prescribing in outpatients from the Second Hospital of Tianjin Medical University, and analyze the shortcoming and deficiency compared with 2010 Chinese guidelines for the management of hypertension. Methods A total of 154 262 electronic prescribing for outpatients with hy-pertension, from January-December 2012 in a Grade 3A hospital in Tianjin, were enrolled in this retrospective survey. Data of commonly used antihypertensive medication and combination therapy in patients were analyzed. The patient data collected were divided into different groups according to age, gender, high blood pressure level and the onset of the season. Results (1)The list of the drugs commonly used for treating hypertension in outpatients were calcium antagonist (52.3%), angiotensin receptor blockers (34.0%),βblockers (25.9%), angiotensin-converting enzyme inhibitors (12.1%), fixed-dose combination (11.0%) and diuretics (1.4%).(2)The fewer combination therapy was found in outpatients than that of monotherapy (43.9%vs 56.1%). Some prescriptions were not routinely recommended by the Guideline (4.6%).(3)The combination therapy used in patients with stage 3 hypertension was higher than that of patients with stage 1or stage 2 hypertension (44.5%vs 37.7%vs 37.7%, P<0.01). The rate of combination therapy was significantly higher in cardiology department than that of other clini-cal departments (P<0.01). The combination therapy tended to be used in the elderly patients than that of non-elderly pa-tients (P<0.01). The number of prescriptions was lower in summer than that of other seasons,but the rate of combination therapy was higher in summer than that of spring, autumn and winter (P<0.01). Conclusion The prescriptions of combina-tion therapy and diuretic were inadequate in outpatients with hypertension. These findings indicate the difference between clinical prescription and the guideline for the management of hypertension.

A Rapid Filtration Sampling Probe for Fermentation Broth On-line Measurement / 分析化学

An inner-bioreactor rapid filtration sampling probe was designed to resolve the problems of continuous sampling the fermentation broth in on-line measurement the fermentation process. The probe consists of a support, a plug valve, an O-ring, a filter membrane and a pipe cover. It can be inserted directly into the bio-reactor withstanding high-temperature sterilization before fermentation, or preventing the bacteria's invasion during sampling. Fermentation broth in tank is cross-flow filtrated by a cylindrical ceramic membrane which is used as filtration membrane, and then the sterile permeated liquid is transported to the analyzer by a peristaltic pump. The sampling experiments using this device was described for sampling glucose solution indicating it is suitable for fermentation online sampling. The sampling rate is up to 3. 0 mL/ min, glucose permeability maintains 100% , and the delay time caused by the probe is about 2 min.